thoughtvova.blogg.se - Liquid liquid extraction in downstream processing

Microbes + fat Lactose Protein Water Salts Nanofiltration (200 - 700 MW) Reverse Osmosis (<200 MW) Ultrafiltration (2000 - 50,000 MW) Microfiltration (100,000 MW) Membrane Fraction Technologies

Concentration polarization and membrane fouling.

Membrane Separation Function : clarification and sterilization Purification of Lactic Acid from Fermentation Broths by Ion-Exchange Resins First, a strong cation resin was used to reduce the broth pH and remove the cations present in the solution Then the acidified broth was purified by an anion exchanger. Organic Resin Groups Linkage group Cation exchange Chloride Anion exchangeĮ.g.

Functional group for complexing anion or cation.

Polymer backbone not involved in bonding.

Organic or inorganic polymer used to exchange cations ( positive ions)or anions ( negative ions)from a solution phase.

Acidifying (liberate the citric acid from its salt ).

Neutralizition (forming calcium citrate ).

Citric acid extraction and purification process.

Insoluble salt precipitation Usually used in extraction of amino acid and organic acid. Organic solvent precipitation By adding an organic solvent to an aqueous fermentation broth, the dielectric constant will decrease causing the solubility to decrease.Įxample Xanthan gum is recovered from an aqueous fermented broth containing the gum by adding to the broth organic solvent.ģ. Isoelectric Precipitation GA+ GA± GA– GA= pH 2 3.22 7.0 >12 pIĢ.

Solid formed is called the precipitateand the liquid remaining above the solid is called the supernatant.ġ.Formation of a solid in a solution during a chemical reaction.Reducing the volume of material to be handled and concentrating the product.Flocculation: Forming woolly cloudlike aggregations. Less-dense components migrate towards the axisĬoagulation and Flocculation Coagulation: Forming semisolid lumps in a liquid.

More-dense components migrate away from the axis of the centrifuge.Use of the centrifugal force for the separation of mixtures.Type of filtration unit Plate and frame filter Typical Operations Filtration A mechanical operation used for the separation of solids from fluids. Removal of insoluble's Capture of the product as a solute in a particulate-free liquid Example Separation of cells, cell debris or other particulate matter from fermentation broth containing an antibiotic. 4.Product preparation (crystallization, drying).Removing components with properties similar to.Purification (chromatography, ultrafiltration) Removing components with properties significantly ģ.Primary isolation (adsorption, precipitation) Such as organic solvents, volatile flavors and aromas.Ĭell removal (filtration, centrifugation, E N D - Presentation Transcriptĭownstream processing, what and why Downstream processing is any treatment of culture broth after fermentation to concentrate and purify products.Īn ideal bioseparation process should combine high throughput with high selectivity, and should ensure stability of product.įermentation Drug producing Happy growing Nutrients Oxygen Genetic modification Microbes Downstream Processing Smash the microbes Remove cells/debris Kill the microbes Concentrate and Purify the product Formulate product Marketĭistillation Separating components with different volatilities.